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Hormone Test

General Information About the Test

Hormone tests are biochemical blood tests that measure the levels of chemical messengers produced by endocrine glands—such as the thyroid, pituitary, adrenal, and reproductive glands (ovaries/testes)—which mix with the blood to regulate body functions. They are requested by endocrinologists and gynecologists/urologists to find the underlying causes of infertility, menstrual irregularities, thyroid diseases, excessive hair growth, menopause, chronic fatigue, and developmental delays.

Test Process and Preparation

Hormone testing process and blood draw timing.
Is fasting mandatory for hormone tests?

Because most hormones (especially TSH, Cortisol, Prolactin, and Insulin) are affected by daily diet and stress factors, it is generally preferred that they be given between 08:00 - 10:00 in the morning, after a full overnight fast.

When are reproductive hormones measured in women?

For the most accurate evaluation of female hormones such as FSH, LH, and Estradiol (E2), it is very important that the blood sample is given on the 2nd or 3rd day of menstrual bleeding.

What should be considered before the test?

Hormones like prolactin and cortisol are highly sensitive to stress. You should get a good night's sleep before giving blood, avoid excessive exercise, and rest by sitting for 10-15 minutes before having your blood drawn when you arrive at the hospital in the morning.

The information on this page is for informational purposes only. The results of medical tests must be evaluated by specialist physicians.


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